Handbook of Histology Methods for Bone and Cartilage > Surgical Pathology

The first comprehensive collection of all the major methods for studying the histology and histomorphometry of bone and cartilage.
The detailed methods include techniques for bone labeling, bone sectioning, histological analysis, histomorphometric analysis, radiological examination, and contact radiography. Among the applications presented are in situ hybridization of bone and cartilage, radiological examination of calcified tissue with emphasis on bone, and dealing with histology artifacts in hard tissue research.
More practical than theoretical, the text is simple and straightforward, with many tables, line drawings, and micro and macro photographs to illustrate the content. Full bibliographies at the end of each chapter guide the reader to additional detailed information.

1
Introduction to Experimental Bone and Cartilage Histology
3
2
Cell Structure and Biology of Bone and Cartilage
35
3
Normal Structure and Function of Bone
59
4
Structure and Function of Articular Cartilage
73
5
Bone-Labeling Techniques
99
6
Human Bone Biopsy
119
7
Biopsy Issues for Bone and Cartilage Tumors
129
8
Tissue Harvesting and Fixation
143
9
Common Fixatives of Hard-Tissue Histology
159
10
Decalcification of Bone Tissue
167
11
Principles of Embedding and Common Protocols
185
12
Infiltration Techniques and Results in Different Types of Resin
199
13
Histological Techniques for Decalcified Bone and Cartilage
209
14
Techniques for Sectioning Undecalcified Bone Tissue Using Microtomes
221
15
Cutting and Grinding Methods for Hard-Tissue Histology
233
16
Bone Sectioning Using the Exakt System
243
17
Bone Sectioning Using a Modified Inner Diamond Saw
253
18
Bone Sectioning Using the Precise 1 Automated Cutting System
265
19
Bone, Cement, and Metal Implant Interface Preparation with a High-Pressure Water Cutter
271
20
Basic Staining and Histochemical Techniques and Immunohistochemical Localizations Using Bone Sections
281
21
Basic Staining Techniques for Cartilage Sections
287
22
Histochemical and Immunohistochemical Staining of Cartilage Sections
295
23
Staining Techniques for Plastic-Embedded Specimens
315
24
In Situ Hybridization of Bone and Cartilage
321
25
Bone Histomorphometry: Concepts and Common Techniques
331
26
Histological Analysis of Bone-Implant Interface
353
27
Histomorphometric Analysis of Bone-Cement and Cement-Metal Interface
361
28
Histologic Analysis of Bone Healing
375
29
Histomorphometry of Metabolic Bone Conditions
391
30
Histology of Articular Cartilage Repair
411
31
Histological Analysis of Cartilage Conditions
423
32
Histological Analysis of Soft-Tissues Biomaterial Interface: Relevance to Dental Implants
439
33
Pathological Diagnosis of Common Tumors of Bone and Cartilage
447
34
Methods for Transmission and Scanning Electron Microscopy of Bone and Cartilage
497
35
SEM Methods for Observation of the Bone-Implant Interface
505
36
TEM Methods for Observation of the Bone-Implant Interface
513
37
Radiological Examination of Calcified Tissues with Emphasis on Bone
531
38
Microangiography, Macrosectioning, and Preparation for Contact Radiography
549
39
Recognizing and Interpreting Histology Artifacts in Hard-Tissue Research
561
Index
571

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