Neural Stem Cells: Methods & Protocols > Cell Biology

Hardcover - 408 pages .
Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.

Preface v
Contributors xi
Introduction
Definitions and Criteria for Stem Cells Leslie P. Weiner 3
Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard Jaime Imitola Samia J. Khoury 9
Isolation and Culture of NSCs
Neural Differentiation of Human Embryonic Stem Cells Mirella Dottori Martin F. Pera 19
Generating Neurons from Stem Cells Andreas Androutsellis-Theotokis Sachiko Murase Justin D. Boyd Deric M. Park Daniel J. Hoeppner Rea Ravin Ronald D. G. McKay 31
Isolation of Neural Stem and Precursor Cells from Rodent Tissue Yuanyuan Wu Ying Liu Jonathan D. Chesnut Mahendra S. Rao 39
Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization Lamya S. Shihabuddin 55
Adult Neural Stem Cells Rossella Galli Angela Gritti Angelo L. Vescovi 67
Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb Susan C. Barnett A. Jane Roskams 85
Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium Miranda Richter Kathryn Westendorf A. Jane Roskams 95
Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property Seung U. Kim Atsushi Nagai Eiji Nakagawa Hyun B. Choi Jung H. Bang Hong J. Lee Myung A. Lee Yong B. Lee In H. Park 103
Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood Shijie Song Juan Sanchez-Ramos 123
Production of Neurospheres from CNS Tissue Gregory P. Marshall II Heather H. Ross Oleg Suslov Tong Zheng Dennis A. Steindler Eric D. Laywell 135
Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells Shigeo Saito Ken Sawai Yoshinobu Murayama Keiichi Fukuda Kazunari Yokoyama 151
In Vitro Studies: A. Cellular Techniques
Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures Angela Gritti Rossella Galli Angelo L. Vescovi 173
Telomere Neurobiology Mark P. Mattson Peisu Zhang Aiwu Cheng 185
Chromosomal Mosaicism in Neural Stem Cells Suzanne E. Peterson Jurjen W. Westra Christine M. Paczkowski Jerold Chun 197
In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype Marcel M. Daadi 205
In Vitro Migration Assays of Neural Stem Cells Pascale Durbec Isabelle Franceschini Francoise Lazarini Monique Dubois-Dalcq 213
Measuring Apoptosis in Neural Stem Cells Brett T. Lund Eve E. Kelland 227
Markers of Adult Neural Stem Cells Eric Wexler 243
In Vitro Studies: B. Molecular Techniques
Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR Nicole Slawny Crystal Pacut Theresa E. Gratsch 271
Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells Yan Chen Jiang F. Zhong 293
Electroporation Strategies for Genetic Manipulation and Cell Labeling Terence J. Van Raay Rhonda T. Lassiter Michael R. Stark 305
Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches Luciano Conti Elena Cattaneo 319
Studies for Transplantation
Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo Sanjay S. Magavi Jeffrey D. Macklis 335
Immunocytochemical Analysis of Neuronal Differentiation Sanjay S. Magavi Jeffrey D. Macklis 345
Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold Lisa A. Catapano Sanjay S. Magavi Jeffrey D. Macklis 353
Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS Qi-lin Cao Stephen M. Onifer Scott R. Whittemore 361
Optimizing Stem Cell Grafting into the CNS Scott R. Whittemore Yi Ping Zhang Christopher B. Shields David S. K. Magnuson 375
Routes of Stem Cell Administration in the Adult Rodent Alison E. Willing Svitlana Garbuzova-Davis Paul R. Sanberg Samuel Saporta 383
Index 403

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